Melatonin alleviates hypertrophic cardiomyopathy. (IMAGE)
Caption
(A) The green intensity of cell ROS was measured by flow cytometry in MYBPC3-KO hiPSC-CMs at day 35, in comparison to WT hiPSC-CMs (n = 3; 10,000 cells per sample). (B, C) Quantification of peak, time to peak, and calcium transient duration in WT and MYBPC3 KO hiPSC-CMs at day 35 (n = 12 cells per group). (D) Quantitative real-time PCR of NPPA and NPPB in WT, MYBPC3-KO, and MYBPC3-KO + melatonin hiPSC-CMs at day 40. (E, F) Cell ROS Green and MitoSOX Red were measured using flow cytometry to assess fluorescence intensity in WT, MYBPC3-KO, and MYBPC3-KO + melatonin hiPSC-CMs at day 40. (G) The peak, time to peak, and calcium decay time were measured in WT-GCaMP, MYBPC3-KO-GCaMP, WT + melatonin, and MYBPC3-KO-GCaMP + melatonin hiPSC-CMs at day 40 (n = 12 cells per group). (H) Quantification of amplitude, time to peak, and relaxation time at day 40 (n = 12 cells per group). (I) Representative western blot of oxidative stress pathway signaling at day 40. *P < 0.05; **P < 0.01; n.s. means no significance. **P versus the corresponding WT group; #P versus the KO group. MYBPC3, myosin binding protein C3; KO, knockout; hiPSC, human induced-pluripotent stem cell; CM, cardiomyocyte; NPPA, natriuretic peptide A; NPPB, natriuretic peptide B; GCaMP, green fluorescent calcium-modulated protein; ROS, reactive oxygen species.
Credit
Jinhua Cao, Yafei Zhai, Ke Li, Jiajv Li, Xiaoxu Tian, Jianchao Zhang, Shuang Li, Mengduan Liu, Xiaowei Li, Jianzeng Dong, Xiaofang Wang
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