MYBPC3 deficiency causes excessive oxidative stress. (IMAGE)
Caption
(A, B) The green intensity of cell ROS was measured by flow cytometry in MYBPC3-KO hiPSC-CMs at day 30 and 40, alongside WT hiPSC-CMs (n = 3; 10,000 cells per sample). (C) A comparison was made between the green intensity of ROS in MYBPC3-KO hhiPSC-CMs and WT hiPSC-CMs at day 30 and 40. Scale bar, 50 μm. (D) GPx level was significantly decreased at day 40 in MYBPC3-KO and WT hiPSC-CMs. (E) Western blotting and quantification of oxidative stress pathway signaling (PI3K, p-PI3K, AKT, p-AKT, FOXO3a, p-FOXO3a) in WT and MYBPC3-KO hiPSC-CMs at day 40. *P < 0.05; **P < 0.01; n.s. means no significance. hiPSC, human induced-pluripotent stem cell; CM, cardiomyocyte; MYBPC3, myosin binding protein C3; KO, knockout; GPx, glutathione peroxidase; PI3K, phosphatidylinositol-3-kinase; AKT, protein kinase B; FOXO3a, forkhead box O 3a; ROS, reactive oxygen species.
Credit
Jinhua Cao, Yafei Zhai, Ke Li, Jiajv Li, Xiaoxu Tian, Jianchao Zhang, Shuang Li, Mengduan Liu, Xiaowei Li, Jianzeng Dong, Xiaofang Wang
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