Col10a1, COX-2, and miRNA-101a expression after intra-articular injection of miRNA-101 agomir in DMM-induced OA mice. Image Caption: (A–C) The relative mRNA (IMAGE)
Caption
(A–C) The relative mRNA expression levels of miRNA-101, Col10a1, and Cox-2 in mouse cartilage at 2, 4, and 6 weeks after intra-articular injection of miRNA-101 agomir (n = 2 in each group). β-actin and U6 were used as endogenous controls.
(D) The immunohistochemical staining of COL10A1 in the articular cartilage tissue of the knee joint.
(E) Quantification of the percentage of COL10A1-positive cells in the three groups as determined by the staining results.
(F) The immunohistochemical staining of COX-2 in the articular cartilage tissue of the knee joint (n = 4 in each group). Magnification, 100× or 400×.
(G) Quantification of the percentage of COX-2 positive cells in the three groups as determined by the staining results. *P < 0.05, **P < 0.01, and ***P < 0.001.
Credit
Rui Mi, Jinnan Chen, Tianxiang Zhu, Huiqin Bian, Rong Wei, Rushuang Deng, Tiaotiao Han, Qian Wan, Yaojuan Lu, Longwei Qiao, Yuting Liang, Qiping Zheng
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND