Intra-articular injection of miR-101a attenuated OA progression. (IMAGE)

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Intra-articular injection of miR-101a attenuated OA progression.

Caption

(A) The diagram illustrates the destabilization of the medial meniscus (DMM) surgical process. Six-week-old male C57BL/6J mice underwent DMM surgery on the medial meniscus of the left hind limb. The medial meniscus tibial ligament, with a portion of the medial meniscus, was dissected by microsurgery. PCL, posterior cruciate ligament; ACL, anterior cruciate ligament. DMM surgery is indicated by blue lightning.

(B) The graphic demonstrates the timeline of the postoperative injection procedure of the three groups of mice. Thirty-three model mice were randomly allocated into three groups (n =11 in each). After three weeks of surgery, mice were treated with equal amounts (15 μL) of normal saline (sham/saline), miRNA control (5 nmol), and miRNA-101 agomir (5 nmol) via intra-articular injection administered through a medial parapatellar approach (the mice in the sham group underwent sham surgery on the right knee). Mice were sacrificed at 2, 4, 6, and 8 weeks after injection for corresponding molecular biological and histological analyses.

(C) The hematoxylin and eosin staining of the knee joint coronal sections. Magnification = 100× and scale bar = 200 μm; magnification = 400× and scale bar = 50 μm. 

(D) The statistical count of the chondrocyte number was determined by hematoxylin and eosin staining (thick red arrow: from the cartilage surface to the deep zone).

(E) The safranin O/fast green staining of the knee joint coronal sections. Magnification = 100× and scale bar = 200 μm; magnification = 200× and scale bar = 100 μm.

(F) The knee joint cartilage thickness was determined by safranin O/fast green staining (black double arrow: from the cartilage surface to the deep zone). *P < 0.05, **P < 0.01, and ***P < 0.001.

Credit

Rui Mi, Jinnan Chen, Tianxiang Zhu, Huiqin Bian, Rong Wei, Rushuang Deng, Tiaotiao Han, Qian Wan, Yaojuan Lu, Longwei Qiao, Yuting Liang, Qiping Zheng

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