Calcium imaging of a live brain slice during clearing with SeeDB-Live (VIDEO)

Kyushu University

Caption

Time-lapse two-photon imaging of the calcium indicator, GCaMP6f, in an acute slice of a 11-day-old mouse olfactory bulb (depth, 150 μm). GCaMP6f was expressed in mitral cells. The medium was exchanged from artificial cerebrospinal fluid (ACSF) to SeeDB-Live/ACSF. The fluorescence of GCaMP6f gradually increased, enabling calcium signal recordings from neurons at tissue depths that are typically difficult to access.

Credit

Shigenori Inagaki and Takeshi Imai, Kyushu University

Usage Restrictions

Credit must be given to the creator.

License

CC BY

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.