Hepatic p62 is dispensable for DDC-diet induced cholestatic injury, fibrosis and hepatocytic MDB formation. (IMAGE)
Caption
The 2–3 months old male liver-specific p62 KO and matched WT mice were fed with DDC for 4 weeks. (A) Representative H&E staining images are shown. Lower panels are enlarged photographs from the boxed areas. Scale bar: 50 µm. Arrows denote porphyrin. Arrowheads denote ductular reactions. (B) Representative Sirius red staining images are shown. (C) Quantification of Sirius red positive areas. Data are presented as mean±SE (n=9–20 images from three to four mice). ***p<0.001, ****p<0.0001, one-way analysis of variance with Tukey post hoc test. Total liver lysates (D–E), detergent soluble (F) and insoluble (G) fractions from mice with the indicated genotype and treatment were subjected to western blot analysis. Data presented as mean±SE from densitometry analysis were presented below each blot. **p<0.01, ***p<0.001, ****p<0.0001 (compared with WT control), #p<0.05 (compared with KO control). One-way analysis of variance with Tukey post hoc test. α-SMA, α-smooth muscle actin; CK8, cytokeratin 8; DDC, 3,5-diethoxycarbonyl-1,4-dihydrocollidine; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; G3BP1, Ras-GTPase-activating protein-binding protein 1; KO, knockout; LC3, light chain 3; Ub, ubiquitin; WT, wild-type.
Credit
By Wen-Xing Ding, Hong-Min Ni, Zhaoli Sun, et al.
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CC BY-NC