Loss of p62 does not affect the recovery of liver injury and MDBs after withdrawal of DDC diet. (IMAGE)
Caption
The 2–3 months old male liver-specific p62 KO and matched WT mice were fed with DDC for 4 weeks and then switched to the Lieber-DeCarli control diet for another 15 days (A). Serum levels of ALT (B), AST (C), ALP (D) and bile acids (E) are quantified. (F) Total liver bile acids are quantified. Data are presented as mean±SE (n=3–4), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, one-way analysis of variance with Tukey post hoc test. (G) Total liver lysates from mice with the indicated genotype and treatment were subjected to western blot analysis. Data presented as mean±SE from densitometry analysis were presented below each blot. ***p<0.001 (compared with WT control), #p<0.05 (compared with KO control). One-way analysis of variance with Tukey post hoc test. Black arrows denote specific bands. (H) Representative H&E and Sirius Red staining images are shown. Scale bar: 50 µm. (I) Quantification of F4/80, MPO and Sirius red positive areas. Data are presented as mean±SE (F4/80, n=46–59 images from three to four mice; MPO, n=42–59 images from three to four mice; Sirius Red, n=15 images from 3 mice). ***p<0.001, ****p<0.0001, one-way analysis of variance with Tukey post hoc test. ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate aminotransferase; α-SMA, α-smooth muscle actin; CK19, cytokeratin 19; DDC, 3,5-diethoxycarbonyl-1,4-dihydrocollidine; EtOH, ethanol; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; KO, knockout; MDBs, Mallory-Denk bodies; MPO, myeloperoxidase; ns, no significance; p-DDC, post-DDC; WT, wild-type.
Credit
By Wen-Xing Ding, Hong-Min Ni, Zhaoli Sun, et al.
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