Increased p62, CK8 and G3BP1 protein aggregation in patients with AH. (IMAGE)
Caption
Representative images of H&E (A) and IHC staining of p62, CK8 and G3BP1 in liver tissues from patients with AH and healthy donors (B). Arrowheads denote pink eosinophilic MDBs in H&E staining; black arrows denote p62 and CK8-positive MDBs, red arrows denote G3BP1-positive SGs. Scale bar: 50 µm. (C) Quantification of IHC staining of p62 (n=11-14 images from three to four patients), CK8 (n=12–13 images from three to four patients) and G3BP1 (n=13–16 images from five to six patients). *p<0.05, **p<0.001. Unpaired Welch’s T test. (D) Representative EM images of a membrane-less inclusion body (arrows). Lower panels are enlarged photographs from the boxed areas. (E–F) Detergent soluble and insoluble fractions from the healthy control and AH livers were subjected to western blot analysis. Data presented as mean±SE (n=5–7) from densitometry analysis were presented below each blot. *p<0.05, ***p<0.001, ****p<0.0001. Unpaired Welch’s T test. Black arrows denote specific bands. Red arrow denotes G3BP1 multimer that was quantified. AH, alcohol-associated hepatitis; CK8, cytokeratin 8; eIF2, eukaryotic initiation factor 2; EM, electron microscopy; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; G3BP1, Ras-GTPase-activating protein-binding protein 1; HC, healthy control; HuR, Hu antigen R protein; IHC, immunohistochemistry; MDBs, Mallory-Denk bodies; ns, no significance; p-eIF2α, phosphorylated eIF2α; SGs, stress granules; TIA-1, T-cell intracellular antigen-1.
Credit
By Wen-Xing Ding, Hong-Min Ni, Zhaoli Sun, et al.
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