The dynamic transition of PF4-associated resident macrophages of the RVes in each group (IMAGE)
Caption
(A) The Monocle prediction of macrophage differentiation and development trajectories based on Seurat clustering information. (B) The Monocle prediction of differentiation trajectories of each macrophage subpopulation. Each dot represents a cell, different colors represent different samples, and the numbers on the black dots merely represent nodes and have no practical significance. (C) The heatmap of the top 50 DEGs along with the pseudotime. The potential fate of individual subsets across pseudotime is illustrated below. Gray represents the starting position of differentiation, namely a branch (State) near the branch node and the developmental origin, namely the branch with small pseudotime; cell fate 1 represents branch node 1, corresponding to branch with small State value, and cell fate 2 represents branch with large State value for analysis node 2. (D) The representative contour plots showing the expression change of the gene Cmss1 and H2-Aa across pseudotime. n = 3 per group. RVes, right ventricle; HR, hypoxia and room light; PR, physiological normoxia and room light; HI, intense light and hypoxia; DEG, differentially expressed gene; SS_Macro, steady-state macrophage; Mo_Macro, monocyte-derived macrophage; Res_Macro, resident macrophage; Pro_Macro, proliferating macrophage; Res_PF4+_Macro, PF4+ resident macrophages; Res_PF4−_Macro, PF4− resident macrophage. PF4, platelet factor 4; H2-Aa, histocompatibility 2 class II antigen A; Cmss1, Cms1 ribosomal small subunit homolog.
Credit
Dingyuan Tian, Yingzi Pan, Xiaoyue Lai, Xinyu Bao, Pan Zheng, Yan Tan, Chun Liu, Ziyang Wang, Qingyuan Yang, Yang Liu, Xiaoqin Wan, Zhihui Zhang, Fang Deng
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CC BY-NC-ND