Genetically knockdown of UCP2 up-regulated BCAA in acute myeloid leukemia. (IMAGE)
Caption
THP-1 or KG-1 cells overexpressing either a scramble control, shUCP2#2, or shUCP2#3 stable transfectants were subjected to RNA-sequencing analysis. (A) KEGG pathway analysis of the top signaling pathways that were aberrantly regulated in the above stable transfectants (∗common signaling pathways in both cell lines). THP-1 or KG-1 cells overexpressing either a scramble control, shUCP2#2, or shUCP2#3 stable transfectants were subjected to metabolic mass spectrometry. (B) Heatmap of the expression profile of amino acid metabolism-related gene expression. (C–E) The peak area for the fold chain of amino acid gene expression (data were normalized to scramble control). (F, G) The BCAA content of THP-1 or KG-1 cells overexpressing either a scramble control, shUCP2#2, or shUCP2#3 stable transfectants (data were normalized to the scramble control). All experiments were repeated three times. Data represent mean ± standard deviation from technical triplicates (∗p < 0.01, ∗∗p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.005; two-way ANOVA for scramble control versus shUCP2s). BCAA, branched-chain amino acid; UCP2, uncoupling protein 2.
Credit
Agida Okohi Innocent, Yajie Shen, Yixuan Gao, Ruixin Sun, Kasimujiang aximujiang, Zizhen Xu, Jinke Cheng, Jiao Ma
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