Analysis of monocyte developmental trajectories in sJIA (IMAGE)
Caption
(A) CytoTRACE-based prediction of monocyte subpopulation differentiation potential. (B) Pseudotime trajectory analysis of monocyte subpopulations (Monocle3). The color gradient from deep blue through purple, red, and orange to yellow represents the relative pseudotime values assigned to each cell, with deep blue indicating early pseudotime states and yellow corresponding to later stages of pseudotime progression. The black lines denote the principal graph inferred by Monocle3, which delineates the major developmental trajectories across the UMAP embedding. (C) Pseudotime-based functional trajectory analysis reveals dynamic enrichment of biological pathways across monocyte subclusters. (D) sJIA cell enrichment along the pseudotime trajectory. (E) NOD-like receptor signaling (NLR) activation along the pseudotime trajectory. (F) Expression of the CXCL10 gene in THP-1 cells under different inflammatory stimuli in vitro. (G) Expression of the CXCL10 protein in THP-1 cells under different inflammatory stimuli in vitro.
Credit
Qiang Luo, Jun Yang, Haiguo Yu, Han Hao, Xinglin Wu, Xiwen Luo, Mingsheng Ma, Xi Yang, Zhiyong Zhang, Yunfei An, Xiaodong Zhao, Hongmei Song, Xuemei Tang
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND