Single-cell atlas of PBMCs from patients with sJIA and controls (IMAGE)
Caption
(A) UMAP visualization of integrated scRNA-seq data, with major immune cell types annotated, including monocyte, NK cell, B cell, plasma cell, megakaryocytes, dendritic cell, and T cell. (Top) sJIA vs. control groups. (Bottom) sJIA-internal, sJIA-external, and sJIA-after-treatment vs. control groups. (B) Dot plot illustrating the expression of canonical marker genes used to annotate major immune cell populations, including monocyte, NK cell, B cell, plasma cell, megakaryocytes, dendritic cell, and T cell. The dot size represents the proportion of cells expressing the gene; the color intensity reflects the average expression level. (C) Radar chart showing the distribution of immune cell types in sJIA and controls. (Top) sJIA and control. (Bottom) internal, external, post-treatment sJIA subgroups and controls. (D) Dot plot showing the Spearman's correlations of monocyte and clinical features across the Chongqing Clinical Dataset (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001). Abbreviations: PLR, platelet-to-lymphocyte ratio; AISI, aggregate index of systemic inflammation; SIRI, systemic inflammation response index; NLR, neutrophil-to-lymphocyte ratio; CRP, C-reactive protein; SII, systemic immune-inflammation index; ESR, erythrocyte sedimentation rate. (E) Dot plot showing inflammatory cytokine expression across immune cell types in the multicenter sJIA cohort.
Credit
Qiang Luo, Jun Yang, Haiguo Yu, Han Hao, Xinglin Wu, Xiwen Luo, Mingsheng Ma, Xi Yang, Zhiyong Zhang, Yunfei An, Xiaodong Zhao, Hongmei Song, Xuemei Tang
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND