PLXNC1 promotes tumor growth and metastasis in vivo (IMAGE)
Caption
(A) Magnetic resonance imaging (MRI) assessment of wild-type (WT) mice carrying subcutaneous tumors. (B) Tumor volumes calculated by MRI. The tumor volume = area of tumor in each slice × slice thickness. (C) Representative images of tumors from WT mice after subcutaneous inoculation of SL4 cells infected with the control vector or sh-PLXNC1 vector. (D) Mice were killed 14 days following subcutaneous injection, and tumors were dissected and weighed. (E) Bioluminescence images showing tumor metastasis by tracking luciferase-expressing SL4 cells in sh-NC and sh-PLXNC1 groups. (F) Histogram showing the bioluminescent signal intensity analyzed by the IVIS System. (G) MRI inspection of hepatic tumor metastasis of colon cancer after intrasplenic injection of SL4 cells in sh-NC and sh-PLXNC1 groups. (H) Gross examination of hepatic tumor metastasis of colon cancer after intrasplenic injection of SL4 cells in sh-NC and sh-PLXNC1 groups. (I) Mice were sacrificed on day 10 after intrasplenic injection, and livers were excised and weighed. (J) Immunohistochemical staining was used to detect the expression levels of PCNA, CD31, TGF-β, vimentin, β-catenin, and E-Cadherin in tumor tissues from sh-NC and sh-PLXNC1 groups. Scale bars = 50 μm. (K) Quantification of immunohistochemical staining in subcutaneous tumor tissues from sh-NC and sh-PLXNC1 groups. (L) Immunohistochemical staining showing expression of epithelial–mesenchymal transition (EMT) markers in liver metastasis tumor tissues from sh-NC and sh-PLXNC1 groups. (M) Quantification of immunohistochemical staining in liver metastasis tumor tissues from sh-NC and sh-PLXNC1 groups. ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.05; ns, not significant.
Credit
Weiqi Wang, Mingxuan Zhou, Tiegang Li, Wenqiang Gan, Silin Lv, Zheng Yan, Yufang Hou, Zifan Zeng, Liu Yang, Fang Zhang, Wenyi Zhao, Min Yang
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