ATOX1 exerts a functional role in AD through interaction with ATP7B. (IMAGE)
Caption
(A, B) Expression of the main copper export protein ATP7B was detected in BV2 microglia with or without Aβ1-42 by Western blot analysis. ATP7B protein levels were quantified. (C) Detection of the interaction of ATOX1 and ATP7B on BV2 microglia cells treated with Aβ1-42 by co-immunoprecipitation. (D) Immunofluorescent staining of ATOX1 (magenta), ATP7B (red), IBA1 (green), and DAPI (blue) in the brain from 5-month-old wild-type and 5 × FAD mice. Scale bar: 30 μm. (E) Quantification of ATOX1 and ATP7B intensity in microglia. ∗∗p < 0.01, ∗∗∗p < 0.001; data were reported as mean ± standard error of the mean; t-test and Tukey's post hoc analysis.
Credit
Fuxin Zhong, Jiani Wu, Zhangjing Deng, Wuhan Yu, Jiaqi Song, Yingxi Chen, Weihua Yu, Yang Lü
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