TUBA1A plays a key role in regulating HCC cell motility. (IMAGE)
Caption
(A) Ectopic expression of TUBA1A in CRL8024 was verified by qRT‒PCR (n = 3). (B, C) Representative images and quantification of migration (B) (n = 9) and wound healing (C) (n = 16) induced by the indicated CRL8024 cells. (D) The silencing efficacy of siTUBA1A in CRL8024 was quantified by qRT‒PCR (n = 3). (E, F) Representative images and quantification of migration (E) (n = 5) and wound healing (F) (n = 6) induced by the indicated CRL8024 cells. (G) The silencing efficacy of siTUBA1A in Huh7 was quantified by qRT‒PCR (n = 3). (H, I) Representative images and quantification of migration (H) (n = 5) and wound healing (I) (n = 5) induced by the indicated CRL8024 cells. (J) The mRNA expression of TUBA1A was positively correlated with that of SNAI1, TWIST1, and VIM in the TCGA-LIHC dataset (n = 423). (K) Results of gene set enrichment analysis (GSEA) of TCGA-LIHC mRNA-seq data based on TUBA1A expression. The data are presented as the mean ± SD. P values were computed using the unpaired Student's t-test (A–I), and Pearson's correlation test (J). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
Credit
Liangzhan Sun, Hui Yang, Pengchao Hu, Jingyi Zheng, Yuyang Du, Shasha Wu, Han Gao, Hao Luo, Yanchen Wang, Fenfen Wang, Jingsong Yan, Xin-Yuan Guan , Yan Li
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