ADAR1p110 promotes HCC metastasis. (IMAGE)
Caption
(A) Schematic diagram of breeding liver-specific Adar1p110 knock-in and c-Myc induced orthotopic liver cancer mouse model. (B) Schematic diagram of DEN/CCl4-induced HCC mouse model. (C) Representative image of DEN/CCl4-induced mouse HCC and statistical analysis of tumor numbers (n = 5). (D) Representative image of c-Myc-induced mice HCC and statistical analysis of tumor numbers (n = 5). (E) Volcanic maps of differentially expressed genes in the c-Myc induced mouse HCC model. Red dots: significantly up-regulated in Adar1p110 knock-in mice. Blue dots: significantly down-regulated in Adar1p110 knock-in mice. (F) Results of gene set enrichment analysis (GSEA) of mRNA-seq data from the c-Myc induced mouse HCC model. (G) Results of gene set enrichment analysis (GSEA) of TCGA-LIHC mRNA-seq data based on ADAR1 expression. (H) Representative images and statistical results of the tail vein injection lung metastasis model (n = 5, one mouse in the WT group died due to anesthesia; autopsy confirmed that it had no metastasis). The data are presented as the mean ± SD. P values were computed using the unpaired Student's t-test (C, D) and Exact binomial test (H). ∗P < 0.05, ∗∗P < 0.01.
Credit
Liangzhan Sun, Hui Yang, Pengchao Hu, Jingyi Zheng, Yuyang Du, Shasha Wu, Han Gao, Hao Luo, Yanchen Wang, Fenfen Wang, Jingsong Yan, Xin-Yuan Guan , Yan Li
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND