MiRNA expression in plasma exosomes of chronic liver injury mice. (IMAGE)
Caption
(A) Flow chart of plasma exosomal miRNA sequencing (Created with FigDraw.com). (B) Volume distribution as a function of particle diameter (nm), as measured by nanoparticle tracking analysis. (C) Transmission electron microscope image showing isolated exosomes (highlighted in red circles, scale bar, 100 nm). (D) Heatmap of miRNAs upregulated in plasma exosomes from MCD-treated and CCl4-treated mice compared with their respective controls. (E) Criteria for selecting candidate miRNAs: upregulated in both MCD and CCl4 models, with log2(fold change) >1 and p<0.05. (F) Schematic of the experimental timeline for MCD-fed mice (n=3) and HFD-fed mice (n=4) (Created with FigDraw.com). (G) MiR-802 expression levels in different organs of MCD-fed mice and chow-fed controls (heart, liver, lung, spleen, kidney, n=3). (H) MiR-802 expression levels in different organs of HFD-fed mice and chow-fed controls (heart, liver, lung, spleen, kidney, n=4). (I) MiR-802 expression in plasma exosomes from MCD-fed mice (n=3) versus chow-fed controls (CT, n=5), and from HFD-fed mice versus chow-fed controls (CT, n=6). (J) MiR-802 expression in plasma exosomes (n=9) and liver tissues (healthy, n=4; MASLD, n=5) from patients with MASLD compared with healthy controls. *p<0.05; **p<0.01. CCl4, carbon tetrachloride; CT, control; HFD, high-fat diet; MASLD, metabolic dysfunction-associated steatotic liver disease; MCD, methionine-choline-deficient diet; miRNA, micro RNA.
Credit
By Yingying Liu, Jingjing Liu, Ping Song, et al.
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CC BY