Exercise and TRF suppress miR-802 to ameliorate MASLD in mice. (IMAGE)
Caption
(A) Schematic of the exercise intervention protocol in HFD-fed mice (Created with FigDraw.com). (B) Hepatic and plasma exosomal miR-802 expression in HFD-fed mice under sedentary (Sed, n=5), exercise (Exe, n=7) or exercise combined with AAV8-miR-802 overexpression (Exe+AAV8-miR-802, n=8) conditions. Plasma exosome sample size: n=4 per group. (C) Hepatic TC and TG levels in Sed (n=5), Exe (n=7), and Exe+AAV8-miR-802 groups (n=8). (D) Representative Oil Red O and H&E staining of liver sections from Sed (n=5), Exe (n=7) and Exe+AAV8-miR-802 mice (n=8, scale bar, 200 µm). (E) Schematic of the TRF intervention protocol in HFD-fed mice (Created with FigDraw.com). (F) Hepatic and plasma exosomal miR-802 expression in HFD-fed mice under ALF (n=5), TRF (n=5) or TRF combined with AAV8-miR-802 overexpression (TRF+AAV8-miR-802, n=4) conditions. Plasma exosome sample size: ALF n=3, TRF and TRF+AAV8-miR-802 n=4 each. (G) Hepatic TC and TG levels in ALF (n=5), TRF (n=5) and TRF+AAV8-miR-802 groups (n=4). (H) Representative Oil Red O and H&E staining of liver sections from ALF (n=5), TRF (n=5) and TRF+AAV8-miR-802 mice (n=4, scale bar, 200 µm). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. AAV8, serotype 8 adeno-associated virus; ALF, ad libitum feeding; Exe, exercise; H&E, haematoxylin and eosin; HFD, high-fat diet; MASLD, metabolic dysfunction-associated steatotic liver disease; miRNA, microRNA; ns, no significance; Sed, sedentary; TC, total cholesterol; TG, triglyceride; TRF, time-restricted feeding.
Credit
By Yingying Liu, Jingjing Liu, Ping Song, et al.
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