Hepatocyte lipotoxicity was regulated by miR-802. (IMAGE)
Caption
(A) Schematic of AML12 cell transfection with miR-802 mimics or inhibitors followed by PA-induced steatosis modelling (Created with FigDraw.com). (B) MiR-802 expression in AML12 cells transfected with mimics or inhibitors (n=4) compared with negative control (NC: n=5). (C) TC and TG levels in NC-transfected and miR-802 mimic-transfected AML12 cells after PA induction (n=5). (D) Representative Oil Red O staining of NC and miR-802 mimic-transfected cells after PA treatment (n=6, scale bar, 100 µm). (E) TC (n=4) and TG (n=5) levels in NC-transfected and miR-802 inhibitor-transfected AML12 cells after PA induction. (F) Representative Oil Red O staining of NC and miR-802 inhibitor-transfected cells after PA treatment (n=5, scale bar, 100 µm). (G) Experimental timeline of MCD-fed mice treated with miR-802 sponge or vehicle control, and hepatic miR-802 expression by qRT-PCR (vehicle: n=7, sponge: n=8) (Created with FigDraw.com). (H) Hepatic TC and TG levels in MCD-fed mice treated with vehicle or miR-802 sponge (vehicle: n=7, sponge: n=8). (I) Representative Oil Red O and H&E staining of liver sections from vehicle-treated or miR-802 sponge-treated MCD-fed mice (vehicle: n=7, sponge: n=8, scale bar, 200 µm). (J) Experimental timeline of HFD-fed mice treated with vehicle or miR-802 sponge, and hepatic miR-802 expression (vehicle: n=5, sponge: n=6) (Created with FigDraw.com). (K) Hepatic TC and TG levels in HFD-fed mice treated with vehicle or miR-802 sponge (vehicle: n=5, sponge: n=6). (L) Representative Oil Red O and H&E staining of liver sections from vehicle-treated or miR-802 sponge-treated HFD-fed mice (vehicle: n=5, sponge: n=6, scale bar, 200 µm). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. AAV, adeno-associated virus; AML12, alpha mouse liver 12; H&E, haematoxylin and eosin; HFD, high-fat diet; MCD, methionine-choline-deficient diet; NC, negative control; PA, palmitic acid; TC, Total cholesterol; TG, triglyceride.
Credit
By Yingying Liu, Jingjing Liu, Ping Song, et al.
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CC BY