Overexpression of TYW5 in the mPFC disrupts circadian gene networks and GnRH signaling pathways. (IMAGE)
Caption
(A) The timeline of transcriptomic analysis for TYW5 overexpression in the mPFC of mice (TYW5-OE: n = 4; controls: n = 4). (B) The volcano illustrated that the expression of thirty genes was significantly altered between TYW5-OE mice and controls (P adj < 0.05). (C) Gene Ontology (GO) enrichment analysis categorizes differentially expressed genes (DEGs) into the top 10 terms across biological processes, cellular components, and molecular functions. (D) KEGG pathway analysis reveals significant enrichment (P adj < 0.05) in circadian rhythm, amino acid biosynthesis, GnRH signaling, and MAPK cascades, with color intensity reflecting statistical significance. (E) Protein interaction network (Cytoscape v3.10.2/ClueGo v2.5.10) demonstrating functional clustering of DEGs within circadian regulation, tRNA aminoacylation, and endoplasmic reticulum stress pathways.
Credit
Qing Zhang, Yaqi Zhang, Lili Yu, Ninglin Fan, Xinglun Dang, Danyang Zhou, Yong Wu, Miao Li, Yi Yang, Yingqi Guo, Lu Wang, Yongfeng Yang, Xi Su, Meng Song, Luxian Lv, Wenqiang Li, Yunqiao Zhang, Qing Long, Zhaowei Teng, Yong Zeng, Jingmei Zhong, Chuang Wang, Shiwu Li
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