TYW5 overexpression in the mPFC induces daytime anxiety-like behaviors and nocturnal locomotor hypoactivity. (IMAGE)

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TYW5 overexpression in the mPFC induces daytime anxiety-like behaviors and nocturnal locomotor hypoactivity.

Caption

(A) Schematic timeline of mouse model construction and behavioral tests. (B) The representative image showing the correct AAV injection sites (mPFC), which expresses red fluorescent protein. (C) The validation of TYW5 protein overexpression in the mPFC. n = 2 mice per group. (D) The body weight of TYW5-OE mice was not significantly changed compared with controls. (E) Overview of the elevated O maze test. (F–J) The indices of test mice, including total distance, average speed in the open area, distance in the open area (%), time in the open area (s), and entries into the open area, were evaluated during the elevated O maze test, and TYW5-OE mice exhibited obvious anxiety-like behaviors compared with controls. (K) Alternations (%) were evaluated during Y maze test, and results showed no differences between controls and TYW5-OE mice. (L, M) The immobility time (%) and struggling time (%) were evaluated during the tail suspension test, and there is no obvious difference between TYW5-OE and controls. (N) Overview of prepulse inhibition test. (O, P) There was no significant difference in the startle stimulus and prepulse inhibition (%) between TYW5-OE and control mice. (Q) Overview of voluntary wheel-running test. (R, S) Overexpression of TYW5 in the mPFC of mice significantly induces hypoactivity of mice at ZT13, ZT15, ZT16, and ZT19. Data represent mean ± standard error of the mean (TYW5-OE: n = 11; controls: n = 11) for (D, F–M, O, P), (TYW5-OE: n = 5; controls: n = 6) for (R, S). Two-tailed Student's t-test: (D, F–M, O, R); Two-way ANOVA test: (P, S). A significance level set at P < 0.05.

Credit

Qing Zhang, Yaqi Zhang, Lili Yu, Ninglin Fan, Xinglun Dang, Danyang Zhou, Yong Wu, Miao Li, Yi Yang, Yingqi Guo, Lu Wang, Yongfeng Yang, Xi Su, Meng Song, Luxian Lv, Wenqiang Li, Yunqiao Zhang, Qing Long, Zhaowei Teng, Yong Zeng, Jingmei Zhong, Chuang Wang, Shiwu Li

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