Tyw5 knockout in male mice induces gene dosage-dependent nocturnal locomotor hyperactivity. (IMAGE)
Caption
(A) Schematic timeline of mouse model generation and behavioral tests. (B) Representative images of wild-type (WT), heterozygous (Tyw5-KO-Het), and homozygous (Tyw5-KO-Hom) mice. (C) Body weight remained unchanged across three genotypes, confirming no gross developmental abnormalities. (D) Overview of the elevated O maze test. (E–I) The total distance, average speed in open area, distance in open area (%), time in open area (s), and entries into open area were evaluated during the elevated O maze test, and there was no significant difference among WTs, Tyw5-KO-Het, and Tyw5-KO-Hom mice. (J) Alternations (%) were evaluated during the Y maze test, and revealed that there were no genotype-dependent differences. (K, L) The immobility time (%) and struggling time (%) were evaluated during the tail suspension test, and Tyw5-KO-Hom exhibits antidepressant-like phenotype compared with WTs. (M) Overview of prepulse inhibition test. (N, O) There was no significant difference in the startle stimulus and prepulse inhibition (%) among the three genotypes of mice. (P) Overview of voluntary wheel-running test. (Q, R) Tyw5 knockout mice exhibited gene dose-dependent hyperactivity in 24 h, particularly during the ZT13–ZT15 interval. Data represent mean ± standard error of the mean (Tyw5-KO-Wt: n = 9; Tyw5-KO-Het: n = 9; Tyw5-KO-Hom: n = 11) for (C, E–L, N–O), (Tyw5-KO-Wt: n = 4; Tyw5-KO-Het: n = 4; Tyw5-KO-Hom: n = 4) for (Q, R). One-way ANOVA test for (C, E–L, N, Q); Two-way ANOVA test for (O, R). A significance level was set at P < 0.05.
Credit
Qing Zhang, Yaqi Zhang, Lili Yu, Ninglin Fan, Xinglun Dang, Danyang Zhou, Yong Wu, Miao Li, Yi Yang, Yingqi Guo, Lu Wang, Yongfeng Yang, Xi Su, Meng Song, Luxian Lv, Wenqiang Li, Yunqiao Zhang, Qing Long, Zhaowei Teng, Yong Zeng, Jingmei Zhong, Chuang Wang, Shiwu Li
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