Silencing PTHrP expression attenuated BMP2-induced chondrogenic, hypertrophic, and osteogenic differentiation of mesenchymal stem cells (IMAGE)
Caption
(A) Silencing PTHrP inhibited BMP2-induced Col2a1 gene expression. BMP2 up-regulated Col2a1 gene expression on days 3 (a), 7 (b), and 11 (c), while silencing PTHrP significantly inhibited BMP2-induced Col2a1 expression on days 7 and 11. (B) Silencing PTHrP inhibited BMP2-induced Col10a1 gene expression. BMP2 dramatically up-regulated Col10a1 gene expression on days 3 (a), 7 (b), and 11 (c), while silencing PTHrP significantly inhibited BMP2-induced Col10a1 expression on days 3, 7, and 11. (C) Silencing PTHrP inhibited BMP2-induced aggrecan expression. (a) Alcian blue staining was used to detect Aggrecan production on days 7 and 11. (b) Quantitative analysis also showed that silencing PTHrP significantly inhibited BMP2-induced Aggrecan synthesis on days 7 and 11. (D) Silencing PTHrP inhibited BMP2-induced ALP activity. ALP staining (a) and ALP reading (b) were used to assess ALP activities. Morphological staining on days 7 and 11 was shown (a), and quantitative analysis also showed that silencing PTHrP significantly inhibited BMP2-induced ALP activities on days 7 and 11 (b). ##P < 0.01, ###P < 0.001, and ####P < 0.0001, compared with the control group. ∗P < 0.5, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001, compared with the indicated group.
Credit
Shengqiang Gao, Piao Zhao, Tingting Du, Yu Ye, Shengwen Cheng, Senrui Liu, Chengcheng Du, Tong-Chuan He, Wei Huang, Zhenglin Zhu, Wei Xu, Junyi Liao
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND