Schematic diagram for predicting the therapeutic efficacy (IMAGE)

National Research Council of Science & Technology

Schematic diagram for predicting the therapeutic efficacy

Caption

(A) For the bioorthogonal labeling, unnatural azide groups on the hEPC surface are generated with Ac4ManNAz pretreatment. Then, azide groups of hEPCs are labeled with DBCO-Cy5 via bioorthogonal copper-free click chemistry in vitro (B) Bioorthogonally labeled hEPCs (Cy5-hEPCs) are imaged in hind limb ischemia model via non-invasive FMT for 28 days. The FMT image shows the initial shape of transplanted Cy5-hEPCs is closely correlated to the therapeutic efficacy of stem cells. The condensed round shape of transplanted Cy5-hEPCs can enhance the therapeutic efficacy, compared to spread shape of transplanted Cy5-hEPCs in ischemic site.

Credit

Korea Institute of Science and Technology(KIST)

Usage Restrictions

The sources of photos and research results from KIST must be specified.

License

Licensed content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.