Figure 2 (IMAGE)

Institute for Basic Science

Figure 2

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(a) Schematic representation of a two-enzyme GOx/HRP CEx fusing into FEx. The catalytic reaction was initiated by adding glucose, generating H2O2 by aerobic oxidation, which was then used to oxidize Amplex Red to generate resorufin. (b) Representative fluorescence spectra of resorufin generated by biocatalysis in fused exosomes (FEx-GOx-HRP) at different time points. Reaction rate for the nanoreactor vs glucose concentration to calculate kinetic constants by fitting the Michaelis-Menten equation. (c) Stability and integrity of FEx-GOx-HRP after cellular integration after 48 h. Endosomes (EEA1, red) lysosomes (LAMP1, magenta), and FEx (green) are shown. (d) Plot of Pearson correlation coefficient (PCCLysosome-FEx) values over time for magenta (lysosome) and green (FEx) fluorescence, and for red (Endosome) and green (FEx) fluorescence.

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Institute for Basic Science

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