News Release

Removing globally-used anxiety drug from recycled and wastewater at low cost

Peer-Reviewed Publication

University of Johannesburg

Wastewater and Diazepam (1 of 2)

image: The globally-used anxiety drug Diazepam can now be removed from recycled water and wastewater, using low-cost titanium dioxide nanofibers, says Prof Vinod Gupta from the University of Johannesburg. First marketed as Valium, Diazepam is available in hundreds of brands. The drug is one of three benzodiapezines in the World Health Organisation list of essential medicines, but is also widely abused as an addictive prescription drug. In cities running out of water, removing pharmaceuticals from wastewater in a simple, low cost way is becoming a priority. This 3D image shows Diazepam's molecular structure. view more 

Credit: CC Roberts, Wikimedia at

A low-cost, efficient one-step method to remove the anxiety drug Diazepam from recycled water and wastewater, using titanium dioxide nanofibers has been developed by researchers headed by University of Johannesburg's Prof Vinod Kumar Gupta. Diazepam is used world-wide. It is a member of the benzodiapezine drug group.

Prescription drugs like Diazepam tend to slip through traditional wastewater treatment plants. These plants are not designed to remove the thousands of different pharmaceuticals in use globally. As drug use increases, legal and illegal pharmaceuticals enter the environment through treated sewage and wastewater discharged from drug manufacturing.

"Existing processes that can remove Diazepam and other drugs at large scale from wastewater are expensive, time consuming, inefficient, or all three. Some also consume a lot of energy in multiple steps, or use toxic and hazardous compounds unfriendly to the environment," says Prof Gupta, from the Department of Applied Chemistry at the university.

"Also, Diazepam is not easy to remove from wastewater using traditional methods. It is partially soluble and has a small particle size. For efficient, targeted removal, advanced hybrid nanomaterials are needed," he says.

Used everywhere

Benzodiazepine drugs are used all over the world for anxiety disorders, as anticonvulsants and anti-epileptics, and for terminally ill people as part of essential medicines list from the World Health Organisation (WHO). The WHO list includes Diazepam, first marketed as Valium, as well as Midozolam and Lorazepam. All three are psychoactive drugs, which affect the way a person thinks and feels.

Diazepam, which can be addictive, is available in over 500 brands globally. It has become a widely-abused prescription drug.

In 2017 a team headed by a researcher from New York State University, announced a new method to simultaneously test for 89 legal neuropsychiatric pharmaceuticals and illicit drugs in unfiltered wastewater and freshwater. Diazepam and several other benzodiapezines are included in the list.

Also, in 2017, a review of seven psychoactive drugs, and to what extent they show up in the aquatic environment, was published by Rio de Janeiro State University (UERJ). The review analysed research about Diazepam and five other benzodiapezines.

The researchers found that the presence and concentration of psychoactive drugs (such as Diazepam) is roughly the same in treated wastewater and untreated wastewater. They found that lower-income countries are worse affected. Also, in surface water, psychoactive drugs tested for were "mostly within the range that caused measurable toxic effects in ecotoxicity assays".

The big thirst

As the world rapidly urbanizes, city populations grow significantly in short timescales. In some regions this means that fresh water resources become more constrained. In South Africa, Cape Town residents dread its imminent 'Day Zero': the day the authorities will have to turn off the taps because there will be too little water left in the city's dams and reservoirs.

As a city's options for fresh water become more limited, re-using waste water, even for drinking purposes, becomes a more important option. However, for a city in a developing country, removing pharmaceuticals from waste water needs to happen in an efficient, cost-effective and speedy way. The treated water can then be added back in low volumes to the city's water supply, as is done already in Orange County in California, Singapore and Perth.

Faster, low cost wastewater treatment

"Implementing this water treatment in municipal wastewater treatment plants should be relatively quick and simple," says Prof Gupta.

Titanium dioxide nanofibers remove Diazepam and related drugs in a targeted way, during the photocatalytic decomposition process. The fibers can be used as filters in municipal or industrial treatment plants, he says.

The filter and screen will be made up of hybrid nanomaterials which are highly efficient in the removal of noxious pharmaceutical and other inorganic and organic impurities from municipal and industrial wastewater, says co-author Dr Ali Fakhri from the Department of Chemistry at Islamic Azad University, Tehran, Iran.

"We use a modified hydrothermal manufacturing method, which produces a dense chain network of hollow fibers. The fibers are cross-linked and stable, so there is low risk for fibers to be emitted in the purified wastewater," adds Dr Fakhri.

The nanofibers can be used to remove industrial dyes and other organic pollutants as well. However they need to optimize some important parameters on the fibre structure first, he says.

Concludes Prof Gupa: "The process to make the fibers is also simple and cost-effective. We are planning a pilot plant at the University of Johannesburg to manufacture these nanofibers. Every aspect of the pilot plant is planned to minimize nanofiber pollution to the environment.

"Later the pilot plant will be followed by a demonstration wastewater treatment plant, to show how nanotechnology can remove a range of pharmaceutical impurities efficiently, rapidly, and at low cost."


Corresponding authors Vinod Kumar Gupta (University of Johannesburg, South Africa) and Ali Fakhri (Islamic Azad University, Tehran, Iran) would like to acknowledge financial support from the Ministry of Education and Science of the Russian Federation [Grant No. 16.1384.2017/PCh]

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