Figure 1. Research strategy for identifying changes in brain O-glycosylation in a depression model (IMAGE)
Caption
A mouse model displaying depression-like behaviors was established by exposing animals to chronic variable stress (left). A multi-omics approach was then applied, using high-resolution mass spectrometry to analyze O-glycan composition and protein alterations across nine distinct brain regions (center). This analysis revealed a pronounced reduction in sialylation and downregulation of the glycosyltransferase St3gal1 in the prefrontal cortex, together with associated changes in synaptic proteins and inhibitory neuronal function (right). Through this integrated strategy, the study demonstrated a direct link between brain O-glycosylation abnormalities and depression-like behaviors.
Credit
Institute for Basic Science
Usage Restrictions
Attribution Required
License
Original content