Fig. 2. (IMAGE) Osaka University Caption 3-D imaging of pathological specimens with CUBIC. (Left) The reconstructed 3-D image of a normal human lung tissue stained with green-fluorescent nucleic acid stain SYTO 16 and Alexa Fluor 647-conjugated anti-α-SMA (smooth muscle actin) antibody, showing the network of vasculature in the pulmonary alveolar interstitium. The image was obtained by confocal fluorescence microscopy. (Middle) The reconstructed 3-D image of a lung tissue derived from an amyloidosis patient. The tissue was stained with SYTO 16 and Congo Red, showing the amyloid deposition in artery wall in lung. The image was obtained by confocal fluorescence microscopy. (Right) The reconstructed 3-D image of SYTO 16-stained half-cut human mesenteric lymph node. The image was obtained by light-sheet fluorescence microscopy. Credit 2017 Nojima S. et al. <i>Scientific Reports</i>, 7:9269, 1-14. DOI: 10.1038/s41598-017-09117-0 Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.