Fig.1. (IMAGE) Osaka University Caption Tissue clearing of various human tissues with CUBIC. (Upper) Gross and microscopic images of human lung and lymph nodes cleared by CUBIC. After gross image acquisition at the indicated time points, lung and lymph node tissues were washed with PBS, followed by paraffin embedding, sectioning, and H&E staining. These images show that lung and lymph node tissues were significantly cleared and that CUBIC clearing caused only negligible, if any, degeneration of the tissues. (Lower) Tissue blocks from formaldehyde-fixed normal human lung and lymph node were cut in half. One half was kept in PBS ("Not embedded") whereas another half was first embedded into a paraffin block, was recovered by deparaffinization ("Paraffin-embedded"), and then was subjected to tissue clearing by CUBIC. The gross appearance of the resulting samples was not distinguishable and had an equivalent transparency. Credit 2017 Nojima S. et al. <i>Scientific Reports</i>, 7:9269, 1-14. DOI: 10.1038/s41598-017-09117-0 Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.