High-resolution DNA typing uncovers hidden diversity of Toxoplasma gondii in China
Shanghai Jiao Tong University Journal CenterPeer-Reviewed Publication
A new study published in Science in One Health provides an updated picture of the population genetic structure of the zoonotic parasite Toxoplasma gondii in China. Using high-resolution multilocus sequence typing across 16 genetic markers, researchers analyzed 96 T. gondii DNA samples collected from diverse animal hosts, including pigs, cats, sheep, birds, bats, and captive wildlife, from 12 provinces and regions in China.
The study confirmed that ToxoDB#9, also known as the Chinese 1 lineage, remains the dominant T. gondii genotype in China. However, sequence-based analysis revealed substantial hidden genetic diversity within this apparently clonal lineage that would not be detected by conventional PCR-RFLP genotyping. The researchers also detected the rare ToxoDB#5 lineage, commonly associated with North American sylvatic cycles, in a captive caracal in China, expanding the known geographic occurrence of this lineage.
Population genetic analyses showed high haplotype diversity but limited geographic structuring, suggesting broad dispersal of dominant clonal lineages, likely influenced by host movement and agricultural activities. The findings highlight the importance of systematic molecular monitoring under a One Health framework and support future whole-genome sequencing to clarify isolate origins, transmission routes, and possible recombination among co-circulating lineages.
- Journal
- Science in One Health