Dead or alive, plankton support marine ecosystems
Kyoto UniversityPeer-Reviewed Publication
Kyoto, Japan -- As tiny as they are, marine plankton play pivotal roles in biogeochemical cycles and in sustaining aquatic ecosystems, producing around half of the oxygen on Earth and also acting as carbon sinks. When they die, their decomposing cells produce dissolved organic carbon that can be transformed and stored in the ocean for thousands of years, so understanding plankton mortality is essential for uncovering the mechanisms behind marine ecosystems.
However, assessing the death of a particular plankton population out of a community consisting of hundreds of coexisting species poses a complex challenge. A team of researchers at Kyoto University addressed this by focusing on viral infection and cell lysis, the process through which a cell's membrane breaks down and releases its genetic information into the environment.
First, the team developed a method for measuring plankton lysis by growing cultures of two phytoplankton species, diatoms and raphidophytes, in a seawater-based medium, and then extracting rRNA and quantifying it using digital PCR. Since rRNA released into seawater degrades over time, the researchers added a culture of spike-in ribosomes to the medium and measured the degradation rate constant. The resulting flux model incorporated both changes in host cell-free rRNA and the degradation constant, making it possible to accurately assess the rate of cell lysis.
- Journal
- MicrobiologyOpen
- Funder
- Japan Science and Technology Agency, Japan Society for the Promotion of Science, Kyoto University