21-Feb-2025
New microscope can image, at once, the full 3D orientation & position of molecules in cells
Marine Biological LaboratoryPeer-Reviewed Publication
A hybrid microscope, born at the Marine Biological Laboratory (MBL), for the first time allows scientists to simultaneously image the full 3D orientation and position of an ensemble of molecules, such as labeled proteins inside cells. The research is published this week in Proceedings of the National Academy of Sciences. The microscope combines polarized fluorescence technology, a valuable tool for measuring the orientation of molecules, with a dual-view light sheet microscope (diSPIM), which excels at imaging along the depth (axial) axis of a sample.
- Journal
- Proceedings of the National Academy of Sciences
- Funder
- NIH/National Institute of Biomedical Imaging & Bioengineering, Marine Biological Laboratory, University of Chicago, NIH/National Institutes of Health, Gordon and Betty Moore Foundation, Howard Hughes Medical Institute, Chan Zuckerberg Initiative, Arnold and Mabel Beckman Foundation, U.S. Department of Energy, NIH/National Institute of General Medical Sciences, U.S. National Science Foundation, National Natural Science Foundation of China, Zhejiang University