CRISPR–Cas9-based therapies are widely investigated for their clinical applications. However, there are limitations associated with the strategy, including off-target DNA editing. A group of researchers from Japan has explored a novel strategy involving CRISPR–Cas3 and investigated its potential using a mouse model of transthyretin amyloidosis (ATTR). The results highlight its potential as an efficient genome-editing system. The technology can be developed as a therapeutic strategy for treating ATTR and other genetic disorders.

Infertility is a major healthcare concern. Understanding the molecular regulators governing fertilization, early embryonic development, and implantation is crucial for the success of assisted reproductive technologies. Now, researchers from Kanazawa Medical University integrated one-cell embryo cryopreservation technology, inhibitor library screening, RNA-seq analysis, and CRISPR-Cas9-mediated gene editing to identify eleven novel factors essential for the development of fertilized eggs. The study contributes towards a better understanding of the underlying mechanisms.

Fluorescent dyes enable the visualization of biomolecular localization and dynamics in living systems. To date, no single benzene-based fluorophores with absorption and emission at wavelengths above 600 nm, the ideal wavelength for bioimaging, have been developed. To address this challenge, researchers from Japan have developed bis-pseudoindoxyls. Owing to its unique red-shifted absorption and fluorescence properties, this dye holds promise for applications in red-light-based bioimaging studies.

A six-year study, led by PhD researcher Sarah Watts of the University’s Faculty of Natural Sciences, looked at the impact of deer management on mountain woodland.

A new study offers a solution to infections with the pathogenic fungus Candida auris by taking a new precision diagnostic approach that for the first time enables fast and accurate quantification of C. auris strains from easily obtained swab samples, as well as the quantification of AMR-causing mutations in fungal populations with mixed antifungal susceptibility. The next-generation test builds on previous diagnostic accomplishments of the groups of Wyss Institute Core Faculty members David Walt, Ph.D. and James Collins, Ph.D., who led the effort, and was greatly facilitated by the team’s collaboration with the Wadsworth Center Mycology Lab at New York State Department of Health, which provided a first cohort of patient samples (surveillance swabs) for the team’s initial technology validation.

Researchers at Princeton University and the University of Arizona have quantified U.S. groundwater in unprecedented breadth and detail. Combining direct measurements with artificial intelligence methods, their new map estimates groundwater depth across the continental United States at a resolution of around 30 meters (98 feet). The work provides a foundation for further research as well as local and regional decision-making around irrigation, conservation and water infrastructure.