Immune checkpoint blockade (ICB) therapies have revolutionized cancer treatment, yet many tumors remain resistant. Now, researchers at Korea University identifies the protein kinase WEE1 as a key driver of this resistance. They discovered that, outside its traditional role in the cell nucleus as tumor suppressor, cytoplasmic WEE1 fosters tumor growth and immune evasion by  enhancing AKT hyperactivation. Targeting WEE1 with clinically available inhibitors may re-sensitize tumors to immunotherapy, offering new hope for treatment-resistant cancer patients.

A new study by researchers at Bar-Ilan University has uncovered that certain ocean viruses—specifically RNA viruses—may disrupt how carbon and nutrients are recycled in the ocean, potentially altering the global carbon cycle.

This protocol establishes the chorioallantoic membrane (CAM) model in avian embryos to investigate migrasome-mediated angiogenesis mechanisms. Key methodologies include the CAM nylon mesh assay and ex vivo sprouting assay for quantifying vascular growth, alongside migrasome isolation, labeling, and targeted delivery. CRISPR-generated T4-KO-mCherry-KI embryos enable functional analysis of migrasome dynamics in real-time angiogenesis. The CAM's high vascularity and accessibility allow parallel assessment of pro-/anti-angiogenic factors and migrasome-cell interaction mapping. By integrating genetic editing and migrasome manipulation, this platform bridges migrasome biology with developmental angiogenesis, offering a scalable tool for mechanistic and therapeutic exploration.