Colorless carotenoids have attracted growing interest as naturally derived ingredients for food and cosmetic applications, but their individual geometric isomers have remained difficult to isolate and study. Researchers have now purified high-purity phytoene and phytofluene isomers and comprehensively characterized their structures, ultraviolet absorption, antioxidant properties, isomerization behavior, and skin-related biological activities. Their findings reveal multifunctional properties that may support next-generation skincare, nutricosmetics, and healthy aging products across the food, cosmetic, healthcare, and wellness industries.