Viral-mediated delivery of morphogenic regulators enables leaf transformation in sorghum bicolor (L.)
University of Illinois at Urbana-Champaign Institute for Sustainability, Energy, and EnvironmentRecent advancements in monocot transformation, using leaf tissue as explant material, have expanded the number of grass species capable of transgenesis. However, the complexity of vectors and reliance on inducible excision of essential morphogenic regulators have limited widespread application. Work by researchers at the Center for Advanced Bioenergy and Bioproducts Innovation (CABBI) provides an important proof-of-concept for the use of both altruistic vectors and viral-expressed morphogenic regulators for improving plant transformation. This study showed that these technologies can be combined to increase efficiencies of embryonic calli formation, a limiting step in monocot leaf transformation, to improve protocol efficiencies and reduce experimental complexity. Continued improvement of leaf transformation protocols in monocot species has the potential to impact crop production worldwide.
- Journal
- Plant Biotechnology Journal
- Funder
- U.S. Department of Energy, U.S. Department of Energy