The Korea Food Research Institute (KFRI), a government-funded research institution, collaborates with local distilleries to develop AI models trained on maturation data from traditional Korean spirits.

The hook effect is best explained by how the analyte signal generated from the assay is compromised due to either antibody excess or antigen excess. Reporting false negatives can also impact clinical decisions that may have adverse effects on the patient. The clinical impact of the hook effect will lead to reporting either inaccurately low or false-negative results. Six different patient pools were tested using immunoassay screening methods and also the LC-MS/MS confirmation method. For the immunoassay screening method, 50 ​μL of each patient sample is pooled together for every 100 patient samples. If there is a sample with the hook effect, it would give a very low or a negative result when the pool is tested neat and it would give a positive result when the pool is tested in a 1∶100 dilution. The drugs tested included Cannabinoids, Benzodiazepines, Amphetamines, Ecstasy, EDP, Opiates, Heroin, Cocaine, Fentanyl, Oxycodone and a combined method for Buprenorphine/Norbuprenorphine. Patient Pool number 6 showed a positive Buprenorphine and Norbuprenorphine that was traced back to the sample with the hook effect based on the suggested protocol. The random and different patient pools were prepared by using a Gilson GX241 liquid handler. The neat result for Pool number 6 showed the immunoassay for Buprenorpone and Norpuprenorphine was “Not Detected” at 2.1 ​μg/L while the LC-MS/MS result was 2,780 ​μg/L (cut-off <10 ​μg/L). However, the result for the 1 in 100 dilutions of the pool for the immunoassay was “Detected” at 37 ​μg/L without multiplying by the dilution factor. The result for the LC-MS/MS was 2,970 ​μg/L. The suggested protocol is practical and cost-effective to avoid the clinical impact of reporting either inaccurately low or false-negative results. Also, can be used for testing when suspected samples with the hook effect are investigated.

Abstract

Objective

Multiple reaction monitoring (MRM), is a highly sensitive targeted mass spectrometry technique that is used to validate discovery phase mass spectrometry data. Through this simple technique, relative protein expressions can be acquired without using commercially available antibodies.

Methods

Our previous work showed Gipie silencing of UM-HACC-2A (adenoid cystic carcinoma) cells resulted in upregulation of a plethora of immune proteins in immune cells in 3D ACC - immune co-culture model.

Results

MRM enabled determining relative protein expression of significantly upregulated immune proteins due to silencing of Gipie. Granzyme A, CD48, granzyme B, HLA class I, antigen B, HLA class I, antigen A, galectin 1, vimentin, endoplasmic reticulum chaperone BiP, and dipeptidyl peptidase 1 differential expressions were validated. Whereas coactosin-like protein expressions were found non-significant among control and Gipie silenced ACC.

Conclusions

This manuscript presents the validation data of previous discovery phase mass spectrometry and also accentuate the use of MRM in translational or preclinical studies in the field of biomedical sciences.

Hemophilia is a hemorrhagic disease caused by a lack of clotting factors due to genetic mutations, and is a representative of rare diseases. The prevalence rate of hemophilia is about 2.73/100,000–3.09/100,000 in China, and the absolute number of patients with hemophilia is large given a huge population base. As a part of the Healthy China initiative, prevention and treatment of hemophilia has attracted much attention in the country. With comprehensive promotion of the construction of the grading diagnosis and treatment system for hemophilia, China requires hospitals applying for comprehensive management centers and diagnosis and treatment centers for hemophilia to be able to independently carry out screening and confirming tests related to hemophilia diagnosis. Identifying the type of pathogenic mutation through genetic diagnosis is of great value for assessing the risk of clinical bleeding in hemophilia patients, studying the induction mechanism of clotting factor inhibitors, conducting gene therapy and promoting eugenics. The mutation types of F8 gene are similar in Chinese patients to international data reports. The F9 gene mutations are mainly single-base point mutations, with no mutation hotspots found in Chinese patients. Historically in China, the treatment of hemophilia has gone through insufficient on-demand treatment and exploration of low-dose prophylaxis, and currently, it is moving towards high-dose prophylaxis with better efficacy and individualized prophylaxis, achieving more ideal treatment effects, reducing patient inconvenience and discomfort, and avoiding high treatment costs as much as possible. Based on related registration data and literature, this report comprehensively summarizes the progress and points out the shortcomings in clinical research and medical care as regards hemophilia in China, in the hope of helping further improve the diagnosis and treatment of hemophilia in the country.

Researchers at Xi'an Jiaotong University have made a significant advancement in the development of silicon anodes for lithium-ion batteries (LIBs), introducing a novel binder that dramatically improves the stability and performance of silicon-based electrodes. This breakthrough addresses one of the most critical challenges in silicon anode technology: the unstable solid electrolyte interphase (SEI) layer, which has long hindered the commercialization of high-capacity silicon anodes.

Precise tumor diagnosis and treatment require the support of abundant molecular information. However, conventional molecular diagnostic technologies gradually fail to satisfy the demands of clinical therapy due to limited detection performance. Benefiting from highly specific target sequence recognition and efficient cis/trans cleavage activity, CRISPR/Cas system has been widely employed to construct novel molecular diagnostic strategies, hailed as the “next-generation molecular diagnostic technology”. This review focuses on recent advances in CRISPR molecular diagnostic systems for the detection of tumor variant gene, protein, and liquid biopsy biomarker, and outlines strategies for CRISPR in situ molecular detection. In addition, we explore general principles and development trends in the construction of CRISPR molecular diagnostic system and emphasize the revolutionary impact that it has brought to the field of molecular diagnostics.

This review explores the interplay between COVID-19 and malaria, with a focus on pregnant women. The concurrent infection of SARS-CoV-2 and malaria presents significant challenges in diagnosis, treatment, and management due to overlapping symptoms and complex immune responses. Genetic factors, particularly variations in the angiotensin-converting enzyme 2 (ACE2) receptor, play a crucial role in determining disease susceptibility and severity. Malaria-induced immunomodulation may influence the clinical progression of COVID-19, though the precise impact remains uncertain. The review emphasizes the need for integrated diagnostic and therapeutic approaches to effectively manage co-infections and improve maternal and fetal health outcomes. It underscores the importance of timely intervention and enhanced diagnostic methods to better understand and address these interactions. This review aims to bridge gaps in current knowledge and pave the way for future research and targeted therapeutic interventions, addressing a significant yet often overlooked public health challenge.