Quantum-entanglement-enhanced pH probe SITE-pHorin reveals organelle pH landscape
Peer-Reviewed Publication
Updates every hour. Last Updated: 11-Nov-2025 07:11 ET (11-Nov-2025 12:11 GMT/UTC)
An accurate map of intracellular organelle pH is crucial for comprehending cellular metabolism and organellar functions. However, a unified intracellular pH spectrum using a single probe is still lacking. Now, writing in the journal Science China Life Sciences, a team of researchers from Jian-Sheng Kang developed a novel quantum entanglement-enhanced pH-sensitive probe called SITE-pHorin (single excitation and two emissions pH sensor protein), which features a wide pH-sensitive range and ratiometric quantitative measurement capabilities. They subsequently measured the pH of various organelles and their subcompartments, including mitochondrial subspaces, Golgi stacks, endoplasmic reticulum (ER), lysosomes, peroxisomes, and endosomes in COS-7 cells.
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