Glycosylphosphatidylinositol (GPI) anchoring for cell wall integrity and immune evasion
Peer-Reviewed Publication
Updates every hour. Last Updated: 13-Dec-2025 04:11 ET (13-Dec-2025 09:11 GMT/UTC)
GPI anchoring is indispensable for cell-wall integrity and full virulence of the maize pathogen Cochliobolus heterostrophus. Deletion of ChGPI7 or ChFEM1 crippled appressorium formation, exposes chitin, and triggers host immune detection. A total of 124 potential GPI-anchored proteins were predicted, indicating that this pathway may serve as a potential antifungal target.
Functionalized single-walled carbon nanotubes (SWCNTs) effectively delivered the amh/amhy plasmid into all-female mandarin fish via immersion. At 40 mg/L, plasmid DNA and transcripts were detected in gonads within 7-14 days. By 60-120 days, some fish developed masculinized gonads with downregulated foxl2/cyp19a1a and upregulated amh/dmrt1. The study demonstrates SWCNTs as a viable gene delivery tool in fish and confirms the crucial role of amh/amhy in sex determination via the amhrII/smads pathway activating dmrt1.