Dear Editor,

Nipah virus (NiV) poses a significant threat to global health security due to its high fatality rate and the possibility of human-to-human transmission.1 This zoonotic virus can cause serious effect in both animals and humans.2 Although there are currently no approved vaccines or therapeutics for NiV, there have been promising advances in vaccine development in recent years, bringing us one step closer to curbing the impact of this deadly disease. NiV outbreaks have been reported in several countries in southern Asian countries in support of their statement, outbreaks are taken place annually in Bangladesh.3 The main route of transmission to human is through contact with infected animals, particularly, flying fox (Pteropus) natural reservoir of the virus. However, raising concerns about the pandemic potential of the virus and their impact on human-to-human transmission has also been documented.4

Objective: To retrospectively analyze the expression and significance of γδ T cells and lymphocyte subsets in peripheral blood on patients with multiple myeloma (MM).

Methods: Fifty-five patients with multiple myeloma admitted to Yangpu Hospital Affiliated to Tongji University from January 2022 to August 2023 were selected as the disease group, and were categorized into stage I group, stage II group and stage III group according to the International Staging System (ISS) of multiple myeloma. All included patients were consecutive visits. Another 30 cases of healthy people with physical examination were selected as the control group. Flow cytometry was used to detect the γδ T cells and lymphocyte subsets in the peripheral blood of the subjects. After clinical indicators of patients and healthy controls were collected, the statistical analysis was conducted.

Results: Compared with the control group, the percentage of γδ T cells and CD8+ T cells in the disease group were higher, while, the absolute numbers of total peripheral blood lymphocytes (LYMT) and CD19+ B cells, percentage of CD19+ B cells, CD3+ T cells, CD4+ T cells were lower (P​<​0.05). The percentage of γδ T cells (P​<​0.001), the percentage of CD19+ B cells (P​=​0.003), the absolute number of CD3+ T cells (P​=​0.010), the absolute number of CD8+ T cells (P​=​0.002) were the significant influential factors of MM. γδ T cells (P​=​0.004), neutrophils (P​<​0.001), neutrophil to lymphocyte ratio (P​=​0.003), and white blood cell count (P​<​0.001) were statistically different in different stages. ROC curve suggested that the area under the curve of the percentage of γδ T cells in predicting the MM were 0.973 (P​<​0.001) which had certain diagnostic value.

Conclusions: γδ T cells and lymphocyte subsets (CD8+ T cells, CD19+ B cells, CD3+ T cells, etc.) in patients with newly diagnosis of MM can reflect the immune function of the patient’s organism, which is a key factor of MM. γδ T cells influence the staging of MM patients, and also have certain diagnostic value.

The recent global outbreak of monkeypox (mpox) has underscored the urgent need for rapid, accurate, and accessible diagnostic tools. The unprecedented spread of mpox beyond its endemic regions has highlighted significant gaps in our preparedness for emerging infectious diseases, particularly in diagnostics. Traditional methods like polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), while accurate, often require sophisticated equipment and trained personnel, leading to potential delays in diagnosis and disease management. In response, the development of point-of-care (POC) biosensors has emerged as a critical area of research, offering the promise of rapid, on-site detection with minimal resource requirements. This article explores various biosensing technologies, including CRISPR-based systems, electrochemical sensors, optical biosensors, and microfluidic devices. We discuss the principles behind these technologies, their performance characteristics, advantages, limitations, and potential for real-world application. By leveraging the current state of knowledge, this article provides a comprehensive overview of the latest developments in POC biosensors for mpox detection to enhance cognizance among researchers, healthcare professionals, and policymakers about the latest advancements and opportunities in this critical area of public health, contributing to enhanced global health security and preparedness against mpox and other emerging infectious diseases.

This article presents a novel bi-directional converter based interconnection planning approach for hybrid AC/DC microgrids with high renewable penetration. The study proposes a tri-level planning framework incorporating dynamic converter efficiency and a data-correlated uncertainty set, demonstrating improved cost-efficiency and robustness.