Researchers at the University of Tokyo have demonstrated that the strength of synaptic connections in the cerebral cortex varies during sleep, influenced by synaptic learning rules and neuronal activity. Using simulations, they identified conditions under which “sleep learning” may occur. These findings could deepen our understanding of the relationship between sleep and learning/memory and help elucidate the mechanisms of brain disorders linked to sleep disturbances.

Precise tumor diagnosis and treatment require the support of abundant molecular information. However, conventional molecular diagnostic technologies gradually fail to satisfy the demands of clinical therapy due to limited detection performance. Benefiting from highly specific target sequence recognition and efficient cis/trans cleavage activity, CRISPR/Cas system has been widely employed to construct novel molecular diagnostic strategies, hailed as the “next-generation molecular diagnostic technology”. This review focuses on recent advances in CRISPR molecular diagnostic systems for the detection of tumor variant gene, protein, and liquid biopsy biomarker, and outlines strategies for CRISPR in situ molecular detection. In addition, we explore general principles and development trends in the construction of CRISPR molecular diagnostic system and emphasize the revolutionary impact that it has brought to the field of molecular diagnostics.