This multicenter study investigates the association between hydroxychloroquine (HCQ) dosage and COVID-19 mortality among hospitalized patients in China, aiming to clarify conflicting evidence from prior research. Leveraging data from multiple medical centers, the analysis focuses on determining whether low-dose HCQ confers mortality benefits with acceptable safety, contrasting with potential risks of higher doses. By systematically evaluating clinical outcomes across different HCQ dosage groups, the research seeks to provide evidence-informed guidance for antiviral therapy in COVID-19 management, particularly in resource-constrained settings.

The study aims to uncover the immunological basis of Tanshi (phlegm-dampness) constitution—a subhealth type in traditional Chinese medicine (TCM)—using single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs). TCM categorizes individuals into nine constitutions, with Tanshi linked to metabolic disorders like diabetes and characterized by symptoms such as heaviness, abdominal flabbiness, and oily skin. By analyzing PBMCs from Tanshi and non-Tanshi individuals, the research seeks to bridge TCM constitutional theory with modern molecular immunology, providing a scientific foundation for TCM’s preventive medicine concept.

Adenosine deaminase 2 (ADA2) plays a critical role in immune regulation, particularly in monocyte differentiation and activation. Unlike ADA1, which primarily functions intracellularly, ADA2 can be secreted or trafficked to lysosomes. Deficiency in ADA2 (DADA2) leads to systemic vasculitis marked by elevated TNF-α levels, excessive pro-inflammatory cytokine production, and impaired differentiation of monocytes into anti-inflammatory M2 macrophages. Research demonstrates that intracellular ADA2 localizes within endolysosomes of macrophages, and its reduction correlates with heightened TNF-α secretion. This suggests ADA2 modulates lysosomal adenosine levels, influencing inflammatory pathways. In pneumonia patients, elevated ADA2 levels in bronchoalveolar lavage (BAL) align with increased pro-inflammatory cytokines, while cord blood exhibits low ADA2, fostering an immunosuppressive milieu. Secreted ADA2 binds apoptotic cells, reducing extracellular adenosine and activating immune responses, highlighting its dual intra- and extracellular roles in inflammation.

POSTECH and Linyi University develop ‘SLY,’ a Probe That Glows Yellow Only in Tumor Cells.