Neuroblastoma (NB), the most prevalent extracranial solid tumor in children, poses a significant therapeutic challenge due to its metastasis and high heterogeneity. A recent study leveraging single-cell RNA sequencing (scRNA-seq) has uncovered vital molecular mechanisms underlying NB's progression and metastasis, shedding light on potential therapeutic targets. The research analyzed primary tumors and matched metastases from NB patients, revealing a 'starter' subpopulation of tumor cells responsible for initiating metastasis. By applying various analytical approaches, including evolutionary trajectory analysis and cell-state differentiation prediction, the study delineated the transcriptional landscape of NB and identified a signature associated with poor prognosis.